Difference between revisions of "Part:BBa K2440016"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker. | ||
| − | Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.1 | + | Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.<sup>1</sup> |
| + | It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes.<sup>2</sup> | ||
| − | + | Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients.<sup>3</sup> | |
| − | + | ||
| − | Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL | + | |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 00:46, 1 November 2017
miR-511 target sequence
It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.
Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.1 It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes.2
Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients.3
Sequence and Features
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.