Difference between revisions of "Part:BBa K2348002"
Line 4: Line 4:
  
 
mCardinal obtained from Addgene [https://www.addgene.org/54799/] <br>
 
mCardinal obtained from Addgene [https://www.addgene.org/54799/] <br>
−
This part was used in our acide induced construct [https://parts.igem.org/Part:BBa_K2348011 K2348011]. In this construct it seems like mCardinal won't work in <i>E. coli</i>.
+
This part was used in our acide induced construct [https://parts.igem.org/Part:BBa_K2348011 K2348011]. In this construct mCardinal is expressed but we were unable to detect any flourescence in <i>E. coli</i>. This might be because, as we later noticed, mCardinal is designed for mammalian expression.  
  
 
<br>
 
<br>

Revision as of 18:42, 1 November 2017


mCardinal

mCardinal obtained from Addgene [1]
This part was used in our acide induced construct K2348011. In this construct mCardinal is expressed but we were unable to detect any flourescence in E. coli. This might be because, as we later noticed, mCardinal is designed for mammalian expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 445
    Illegal BsaI.rc site found at 634