Difference between revisions of "Part:BBa K2206000"

Revision as of 22:23, 30 October 2017

Toehold switch for hsa-miR-15b-5p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-15b-5p (the trigger RNA). In the presence of hsa-miR-15b-5p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-15b-5p present in a system.

The transcripts produced by this part are toxic to E. coli. Therefore, we recommend the use of an inducible non-leaky promoter to allow for amplification in E. coli. For reference, we found BBa_K808000 suitable for expressing this part.

See part BBa_K2206006 to see how this part works when regulating GFPmut3b.

This part contains a strong RBS sequence.

NUPACK Structure Analysis

Sequence and Features