Difference between revisions of "Part:BBa K2443013"
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Methionine tRNA synthetase responsible for attaching methionine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
 
Methionine tRNA synthetase responsible for attaching methionine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
  
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<h1>Improved part</h1>
 
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<p class="text12"> <b> Original part:</b> BBa_K567015
 
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                <br><b>Submitted by:</b> SJTU-BioX-Shanghai 2011
 
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                <br><b>Designed by:</b> You Wang
 
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                <br><b>Rational behind improvements:</b>
 
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                BBa_K567015 encodes for a truncated MetRS without the anticodon recognition domain. We have improved this part by including the entire coding sequence, which has been codon optimized for optimal expression in <i>E. coli</i>. For easy purification we have included a C- terminal hexahistidine tag with a serine glycine linker. We have also optimized MetRS to be overexpressed in BL21 DE3 gold cell by putting it under the control of a T7 promoter
 
  
 
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Revision as of 21:47, 30 October 2017


MetRS optimized for expression in E. coli

Methionine tRNA synthetase responsible for attaching methionine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]