Difference between revisions of "Part:BBa I20260"

Latest revision as of 10:12, 1 November 2017

Measurement Kit Test of J23101

Measkit test of J23101

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 706

Characterization IONIS_PARIS 2017

Group & Author: IONIS_PARIS /La Paillasse/2017

Summary: We transformed BBa_I20260 into pSB1C3 from pSB1K3 and sent it to the registry. We thought it was important because the part in PSB1C3 was not available in the registry. This part was very useful as a control for us to characterise BBa_K098997 (pCOLD-GFP) from the iGEM Tokyo 2016 team.

Method: See the bacterial transformation protocol in our wiki IONIS_PARIS 2017_Lab work_protocols.

Results:

The bacteria transformation worked, we had colonies and we then did a PCR colony (Figure 1) to verify if the GFP was transformed into pSBIC3. We picked different colonies and the positive result was observed in the well nº3 (GFP 1). In conclusion the GFP was successfully transformed into pSB1C3.

Figure 1: PCR colony. A) Ladder, B) mRFP protein generator, C) GFP 1, D) GFP 2, E)GFP 3.

@@ Line 21: / Line 21: @@
 Group & Author: IONIS_PARIS /La Paillasse/2017
-Summary: We transformed BBa_I20260 into pSB1C3 from pSB1K3.
+Summary: We transformed BBa_I20260 into pSB1C3 from pSB1K3 and sent it to the registry. We thought it was important because the part in PSB1C3 was not available in the registry. This part was very useful as a control for us to characterise BBa_K098997 (pCOLD-GFP) from the iGEM Tokyo 2016 team.
 Method: See the bacterial transformation protocol in our wiki IONIS_PARIS 2017_Lab work_protocols.