Difference between revisions of "Part:BBa K2271062"
| Line 6: | Line 6: | ||
===Experimental Design an Results=== | ===Experimental Design an Results=== | ||
| − | The part was used to transform <i>S. cerevisiae</i> | + | The part was used to transform <i>S. cerevisiae</i> BY4742. Transformants were used to validate the part by microscopy using the Elyra PS microscope. The microscope images indicating an localisation of mTurquoise-Pex13 in the peroxisomal membrane (Figure 1). |
[[File:Igemduscgn2017pex13Turqoise.png|500px|thumb|center|'''Figure 1''' Richtiges Bild einfügen! The micrscopy showing an localisation of mTurquoise with the typical peroxisomal shape. This indicates, an peroxisomal membrane licalisation of mTurquoise-Pex13]] | [[File:Igemduscgn2017pex13Turqoise.png|500px|thumb|center|'''Figure 1''' Richtiges Bild einfügen! The micrscopy showing an localisation of mTurquoise with the typical peroxisomal shape. This indicates, an peroxisomal membrane licalisation of mTurquoise-Pex13]] | ||
Revision as of 18:02, 30 October 2017
pex13-mTurqoise
Usage and Biology
This part is a composite part comtaining the peroxisomal membrane anchor Pex13 with an N-terminal fusion of the CFP variant mTurquoise. It is applicable for S. cerevisiae using the HHF1 promoter and the TDH1 terminator. The optimal excitation is at a wavelength of 434 nm with an excitation optimum at 474 nm.
Experimental Design an Results
The part was used to transform S. cerevisiae BY4742. Transformants were used to validate the part by microscopy using the Elyra PS microscope. The microscope images indicating an localisation of mTurquoise-Pex13 in the peroxisomal membrane (Figure 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 1987
Illegal BglII site found at 880
Illegal BglII site found at 917 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]