Difference between revisions of "Part:BBa K2286012"
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<partinfo>BBa_K2286012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2286012 SequenceAndFeatures</partinfo>
  
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===Characterization===
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==Characterization==
  
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==Molecular weight==
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===Molecular weight===
  
 
This halohydrin dehalogenase gene codes for a protein of 762 amino acids with a molecular mass of 29kDa[2].
 
This halohydrin dehalogenase gene codes for a protein of 762 amino acids with a molecular mass of 29kDa[2].
  
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[[File:T--UESTC-China--DhaA31-RT-PC.png|500px|thumb|center|'''Fig 5'''.RT-PCR of positive plants.]]
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===Structure of HheC: homologous tetramer===
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It is reported that halohydrin dehalogenase HheC is a symmetric tetramer that can be thought of as a dimer. The four active centers of HheC tetramer are centrosymmetric. The active sites include substrate binding sites and halide binding sites, surrounded by four Loop regions, loop1 (F12-Q87), Loop2 (A133 (373), Loop3 (P175-F188) and Loop4 (F243-P253), while the Ser132-Tyr145-Arg149 catalytic triad is contained in these four Loops. There is a synergistic effect between the amino acid residues on the four Loop regions, which are involved in the change of the active site conformation during the enzymatic catalysis. At the same time, it is reported that the C-terminus of the HheC plays an important role in its catalytic activity and thermal stability [1].
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[[File:T--UESTC-China--HheC-3D-model.png|500px|thumb|center|'''Fig 1'''.3D model of HheC.]]
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===Michaelis constant of HheC toward 2,3-DCP===
  
  

Revision as of 17:56, 30 October 2017


HheC: halohydrin dehalogenase

This is a halohydrin dehalogenase from Agrobacterium radiobacterAD1(HheC). HheC is an important enzyme in the halide dehalogenation reaction, which catalyzes o-halide to epoxides and hydrogen halides through intramolecular nucleophilic substitution mechanism. So it can catalyze many substrates, such as 1,3-dichloro propanol (2,3-DCP), 2,3-dichloropropane-1-ol (2,3-DCP), 3-chloropropane-1,2-diol (CPD) and so on.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 762 amino acids with a molecular mass of 29kDa[2].

Structure of HheC: homologous tetramer

It is reported that halohydrin dehalogenase HheC is a symmetric tetramer that can be thought of as a dimer. The four active centers of HheC tetramer are centrosymmetric. The active sites include substrate binding sites and halide binding sites, surrounded by four Loop regions, loop1 (F12-Q87), Loop2 (A133 (373), Loop3 (P175-F188) and Loop4 (F243-P253), while the Ser132-Tyr145-Arg149 catalytic triad is contained in these four Loops. There is a synergistic effect between the amino acid residues on the four Loop regions, which are involved in the change of the active site conformation during the enzymatic catalysis. At the same time, it is reported that the C-terminus of the HheC plays an important role in its catalytic activity and thermal stability [1].

Fig 1.3D model of HheC.

Michaelis constant of HheC toward 2,3-DCP