Difference between revisions of "Part:BBa K2271118"
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In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast. | In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast. | ||
| − | This part is designed for cytosolic expression, for peroxisomal import please choose part: | + | This part is designed for cytosolic expression, for peroxisomal import please choose part: BBa K2271124 |
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Revision as of 15:33, 30 October 2017
ValS
Valencene synthase converts FPP into Valencene. We chose the ValS from Callitropis nootkatensis, because other valencene synthases perform relatively inefficient in microorganisms. For example, they show a relatively poor product specificity, are less efficient in producing sesquiterpene than other synthases and have a significant amount of side products like germacrene A. In contrast to this, ValS from C. nootkatensis has a good product specificity and is more robust in terms of pH and temperature changes. It has also a better yield than citrus valencene synthases in yeast.
This part is designed for cytosolic expression, for peroxisomal import please choose part: BBa K2271124
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2732
Illegal BglII site found at 860 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]