Difference between revisions of "Part:BBa K2257007"
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FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.<br/> | FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.<br/> | ||
Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.</p> | Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.</p> | ||
| − | [[File:T-Nanjing-China-ch2o-l2.png|600px|thumb|center]] | + | [[File:T-Nanjing-China-ch2o-l2.png|600px|thumb|center]Figure 1. Optical density(600nm) of (a) Escherichia coli BL21 and (b) recombinant bioluminescent Escherichia coli BL21 harboring frmR-RFP fusion after 10 hours’ incubation with 800uM different aldehydes] |
| + | [[File:T-Nanjing-China-ch2o-l1.png|500px|thumb|center]Figure 2. Response growth curve for recombinant bioluminescent Escherichia coli BL21 to different concentration of formaldehyde] | ||
| + | [[File:T-Nanjing-China-ch2o-l3.png|500px|thumb|center]Figure 3. Fluorescence test of various aldehydes using recombinant bioluminescent Escherichia coli BL21] | ||
| + | [[File:T-Nanjing-China-ch2o-l4.png|500px|thumb|center]Figure 4. The tolerance of recombinant bioluminescent Escherichia coli BL21 to various concentration of formaldehyde] | ||
| + | [[File:T-Nanjing-China-ch2o-l5.png|500px|thumb|center]Figure 5. Response fluorescence intensity for recombinant bioluminescent Escherichia coli BL21 to formaldehyde] | ||
| + | ] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:25, 30 October 2017
This part is used for the detection of formaldehyde.
Formaldehyde sensor sequence
Usage and Biology
This protein FrmR acts as regulator of protein FrmAB. FrmA and FrmB are two proteins that are participating in the detoxication of formaldehyde in E.coli.
FrmR can form DNA-protein cross-links with a special promotor PfrmAB to prevent activating downstream gene transcription.
Formaldehyde is able to covalently modify protein FrmR, inhibiting its function, so when formaldehyde exists, promotor PfrmAB is capable of activating downstream gene transcription by the destruction of the promoter PfrmAB- the protein FrmR cross-link.
[[File:T-Nanjing-China-ch2o-l2.png|600px|thumb|center]Figure 1. Optical density(600nm) of (a) Escherichia coli BL21 and (b) recombinant bioluminescent Escherichia coli BL21 harboring frmR-RFP fusion after 10 hours’ incubation with 800uM different aldehydes] [[File:T-Nanjing-China-ch2o-l1.png|500px|thumb|center]Figure 2. Response growth curve for recombinant bioluminescent Escherichia coli BL21 to different concentration of formaldehyde] [[File:T-Nanjing-China-ch2o-l3.png|500px|thumb|center]Figure 3. Fluorescence test of various aldehydes using recombinant bioluminescent Escherichia coli BL21] [[File:T-Nanjing-China-ch2o-l4.png|500px|thumb|center]Figure 4. The tolerance of recombinant bioluminescent Escherichia coli BL21 to various concentration of formaldehyde] [[File:T-Nanjing-China-ch2o-l5.png|500px|thumb|center]Figure 5. Response fluorescence intensity for recombinant bioluminescent Escherichia coli BL21 to formaldehyde] ] Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 226
Illegal XhoI site found at 462 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 520
The sequence is composed of PfrmR, gene frmR, flag tag, PfrmAB, gene RFP.
When the pathway works, we can see that the E.coli turns to red with naked eyes at the presence of formaldehyde.
The fluorescence intensity of protein RFP and the depth of the color in naked eyes are both in positive correlation with the concentration of formaldehyde within the tolerable range.