Difference between revisions of "Part:BBa K2262013"
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<p style="padding:1px;font-size:16px"><b>2. Cloning </b></p>
 
<p style="padding:1px;font-size:16px"><b>2. Cloning </b></p>
  
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We put T7 promoter, RBS, and B-1E together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
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We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
  
  

Revision as of 14:03, 30 October 2017


T7 Promoter+RBS+Pe-4

Figure 1. PT7+RBS+Pe-4+terminator

Introduction

It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.

Experiment

1. Fungal Test


(1) Inhibition Zone


(2) Spore Germination


(3) Botany Experiment

2. Cloning

We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]