Difference between revisions of "Part:BBa K2333011:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The sequence for this part was adapted from Sauer 2008 "Evolution of the ssrA degradation tag in Mycoplasma: specificity switch to a different protease". It was codon-optimized for E. coli.
+
This specific sequence was codon-optimized for E. coli using the website www.jcat.de
  
 
===Source===
 
===Source===

Revision as of 13:29, 30 October 2017


mf-Lon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1834
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 2124
    Illegal AgeI site found at 2149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This specific sequence was codon-optimized for E. coli using the website www.jcat.de

Source

meso

References