Difference between revisions of "Part:BBa K2336036"

Revision as of 12:55, 30 October 2017

PmrC-GFP

This part is used to detect whether the sequence is expressed successfully.

Usage and Biology

PmrC is a promoter used to start the composite. GFP is green fluoresent protein gene.

The aim that we design this part is to get prepared for the material, then PmrA-PmrB(LBT) can be linked to it. Because we need a large amount of this part, we standardize this part independently. Actually, this part is not a functional part. It a basic to get more parts and construct the whole circuit. Figure 1-1 shows the circuit of PmrC-GFP.

Figure 1-1: The circuit of PmrC-GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 397
Illegal XhoI site found at 436
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1162
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1085

@@ Line 9: / Line 9: @@
 <p>PmrC is a promoter used to start the composite. GFP is green fluoresent protein gene.</p>
 <p>The aim that we design this part is to get prepared for the material, then PmrA-PmrB(LBT) can be linked to it. Because we need a large amount of this part, we standardize this part independently. Actually, this part is not a functional part. It a basic to get more parts and construct the whole circuit. Figure 1-1 shows the circuit of PmrC-GFP.
-[[File: CGFP.png|left|thumb|400px|Figure 1-1: The preview of Si-tag displayed on cell wall and work.]]
+[[File: CGFP.png|left|thumb|400px|Figure 1-1: The circuit of PmrC-GFP.]]
 </p>