Difference between revisions of "Part:BBa K2323003"

Revision as of 13:09, 30 October 2017

GFP-pdt2E

This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2E. This causes the protein to degrade in E.coli with a first-order rate of 0.0046 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

This biobrick is part of a collection of degradation tags, characterized on this page. See also BBa_K2323005, BBa_K2323006, BBa_K2323007, BBa_K2323008.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2323003 short</partinfo>
-This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2E. This causes the protein to degrade in E.coli with a first-order rate of ... per min, from the ClpX protease. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.
+This GFP (E0040) is tagged with a protein degradation tag (pdt) called pdt2E. This causes the protein to degrade in E.coli with a first-order rate of 0.0046 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.
+<!-- -->
+This biobrick is part of a collection of degradation tags, characterized on this page. See also [https://parts.igem.org/Part:BBa_K2323005 BBa_K2323005], [https://parts.igem.org/Part:BBa_K2323006 BBa_K2323006], [https://parts.igem.org/Part:BBa_K2323007 BBa_K2323007], [https://parts.igem.org/Part:BBa_K2323008 BBa_K2323008].
 <!-- Add more about the biology of this part here