Difference between revisions of "Part:BBa K2323000"
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Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.  
 
Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.  
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The coding sequence is followed by Tphi terminator.
 
The coding sequence is followed by Tphi terminator.
  
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For characterization, see the full construct: [https://parts.igem.org/Part:BBa_K2323004 BBa_K2323004].
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:41, 30 October 2017


Lwa Cas13a + Tphi terminator

Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.

The coding sequence is followed by Tphi terminator.

For characterization, see the full construct: BBa_K2323004. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365


<Uncomment this to enable Functional Parameter display

Test

Functional Parameters

Test2

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