Difference between revisions of "Part:BBa K2247000"
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This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system. | This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, or simply the addition of galactose, with a higher strength than pGal1. This promoter can serve as an positively regulated promoter in the S. cerevisiae system, or drive the expression of the reporter gene in any standard version of yeast-two-hybrid system. | This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, or simply the addition of galactose, with a higher strength than pGal1. This promoter can serve as an positively regulated promoter in the S. cerevisiae system, or drive the expression of the reporter gene in any standard version of yeast-two-hybrid system. | ||
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| + | ===Characterization=== | ||
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| + | [[File:PGalS-HXT2 glucose medium.jpeg|200px]] | ||
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Revision as of 09:47, 30 October 2017
pGalS promoter of S. cerevisiae
This is a modified version of the yeast endogenous Gal1 promoter, from which expression can be induced by galactose, or the yeast Gal4 activator. pGalS also contains the upstream activating sequence (UAS) which Gal4 can bind, and can serve as the promoter in a standard yeast-two-hybrid system.
Usage and Biology
This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, or simply the addition of galactose, with a higher strength than pGal1. This promoter can serve as an positively regulated promoter in the S. cerevisiae system, or drive the expression of the reporter gene in any standard version of yeast-two-hybrid system.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 453
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 54
- 1000COMPATIBLE WITH RFC[1000]