Difference between revisions of "Part:BBa K1639001"

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Team USP-Brazil 2017 has improved the characterization of this part by expressing the trigger with different concentrations of IPTG and measuring the effect of this indcution on the GFP switch.
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Team [http://2017.igem.org/Team:USP-Brazil USP-Brazil 2017] has improved the characterization of this part by expressing the trigger with different concentrations of IPTG and measuring the effect of this indcution on the GFP switch.
  
 
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<figure>
<https://static.igem.org/mediawiki/2017/8/89/T--USP-Brazil--Detection--RNA1.png">
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<img src="https://static.igem.org/mediawiki/2017/8/89/T--USP-Brazil--Detection--RNA1.png">
 
<figcaption> Induction of trigger RNA in BL21 <i>E. coli</i> cells with different concentrations of IPTG </figcaption>
 
<figcaption> Induction of trigger RNA in BL21 <i>E. coli</i> cells with different concentrations of IPTG </figcaption>
 
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Revision as of 03:15, 30 October 2017

Trigger RNA

This part required for producing reporter flourescence protein GFP from Toehold-GFP(BBa_K1639000) composite part. You can find more details on BBa_K1639000

Usage and Biology

Team [http://2017.igem.org/Team:USP-Brazil USP-Brazil 2017] has improved the characterization of this part by expressing the trigger with different concentrations of IPTG and measuring the effect of this indcution on the GFP switch.

Induction of trigger RNA in BL21 E. coli cells with different concentrations of IPTG
Sequence and Features BBa_K1639001 SequenceAndFeatures