Difference between revisions of "Part:BBa K2262011"
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<h1>'''Experiment'''</h1> | <h1>'''Experiment'''</h1> | ||
| − | < | + | <p style="padding:1px;font-size:16px"><b>1. Fungal Test </b></p> |
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| + | <p style="padding:1px;font-size:14px"><b>(1) Inhibition Zone </b></p> | ||
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| + | <p style="padding:1px;font-size:14px"><b>(2) Spore Germination </b></p> | ||
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| + | <p style="padding:1px;font-size:14px"><b>(3) Botany Experiment </b></p> | ||
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| + | <p style="padding:1px;font-size:16px"><b>2. Cloning </b></p> | ||
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| + | We put T7 promoter, RBS, and B-1E together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p.. | ||
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Revision as of 14:03, 30 October 2017
T7 Promoter+RBS+B-1E
Introduction
It has been known as a antibacterial peptide that has activity against representative Gram-negative and Gram-positive bacterial species. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.
Experiment
1. Fungal Test
(1) Inhibition Zone
(2) Spore Germination
(3) Botany Experiment
2. Cloning
We put T7 promoter, RBS, and B-1E together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]