Difference between revisions of "Part:BBa K2382004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part previously functioned as a DNA recombination and repair protein | + | This part previously functioned as a DNA recombination and repair protein in E. coli. It is also |
| − | in E. coli. It is also found that Thioredoxin is capable of increasing | + | found that Thioredoxin is capable of increasing enzyme activity of our protein, MSMEG5998. |
| − | + | We designed a polylinker that has multiple restriction cutting sites at the end of this part for | |
| − | future | + | future iGEM teams who want to make their protein more effective |
| − | + | ||
===Characterization of the Thioredoxin with polylinker=== | ===Characterization of the Thioredoxin with polylinker=== | ||
Revision as of 03:42, 30 October 2017
Status: 500 Content-type: text/html
Software error:
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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Sequence and Features
Status: 500 Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Usage and Biology
This part previously functioned as a DNA recombination and repair protein in E. coli. It is also found that Thioredoxin is capable of increasing enzyme activity of our protein, MSMEG5998. We designed a polylinker that has multiple restriction cutting sites at the end of this part for future iGEM teams who want to make their protein more effective
Characterization of the Thioredoxin with polylinker
References
Carsten Berndt, Christopher Horst Lillig, Arne Holmgren, Thioredoxins and glutaredoxins as facilitators of protein folding, In Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1783, Issue 4, 2008, Pages 641-650, ISSN 0167-4889, https://doi.org/10.1016/j.bbamcr.2008.02.003. (http://www.sciencedirect.com/science/article/pii/S0167488908000700)
Escherichia coli str. K-12 substr. MG1655 https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3