Difference between revisions of "Part:BBa K2382003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part originated from pET-29 a (+) Vectors and | + | This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, |
| − | We tried to improve this promoter sequence at [https://parts.igem.org/Part:BBa_K525998# BBa_K525998] by adding an lac operator and making it RFC 10 compatible. | + | and RBS. |
| + | |||
| + | We tried to improve this promoter sequence at [https://parts.igem.org/Part:BBa_K525998# BBa_K525998] by adding an lac operator and making it RFC 10 compatible. | ||
===Characterization of the T7 promoter & Lac operator and RBS from PET-29a=== | ===Characterization of the T7 promoter & Lac operator and RBS from PET-29a=== | ||
Revision as of 03:41, 30 October 2017
T7 promoter & Lac operator and RBS from PET-29a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.
We tried to improve this promoter sequence at BBa_K525998 by adding an lac operator and making it RFC 10 compatible.
Characterization of the T7 promoter & Lac operator and RBS from PET-29a
References
pET-29a(+) 5.4kb - GenScript https://www.genscript.com/gsfiles/vector-map/bacteria/pET-29a.pdf