Difference between revisions of "Part:BBa K2271066"

Revision as of 00:35, 30 October 2017

mRuby-ePTS1

Usage and Biology

This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 (Dueber et al.). The part is designed as a peroxisomal lumen marker for S. cerevisiea. using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the an optimal excitation of 559 nm and emission of 600 nm is discriped.

Experimental Design and Results

For the validation the S. cerevisiae Strain BY4247 was tranformed with this part. The cells were fixated and microscope with an xxx microscope. A typical peroxisomal localisation could be validated (Figure 1).

Figure 1 mRuby fused with PTS1 localised peroxisomal typical in the cells

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2271066 short</partinfo>
 ===Usage and Biology===
-The part contains a mRuby fluorezenz protein with an enhanced pts1 sequence, described by Dueber et. Al, with a TDH3 Promotor and a HHF1 Terminator. mRuby is a mutant oft he fluorezent protein gfp with excitation and emission wavelengths at 559 nm/600nm  . With the C-terminal enhanced pts1 (peroxisomal targeting sequnce) the mRuby is imported into the peroxisome. The part can be used as a peroxisomal matrix marker in <i>S. cerevisiea.</i>
+This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 (Dueber et al.). The part is designed as a peroxisomal lumen marker for <i>S. cerevisiea.</i> using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the an optimal excitation of 559 nm and emission of 600 nm is discriped.
 === Experimental Design and Results===