Difference between revisions of "Part:BBa K2213001:Experience"
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===Applications of BBa_K2213001=== | ===Applications of BBa_K2213001=== | ||
| + | To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: | ||
| + | - concentration of Tetracyclin inducer (induces EutMN synthesis) | ||
| + | - concentration of IPTG inducer (induces EutS synthesis) | ||
| + | - Harvest time (time after induction) | ||
| + | - Temperature | ||
| + | - Growth Medium (LB and TB) | ||
| + | / | ||
| + | from this 22 flask investigation we were able to make surface plots to visulise our findings. | ||
| + | / | ||
| + | ***2 TEMPERATURE DoE SURFACE PLOTS*** | ||
| + | / | ||
| + | Figure1: | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:22, 29 October 2017
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Applications of BBa_K2213001
To find the optimal conditions of EutM microcompartment formation we used a tool called 'Design of Experiments' to vary a multitude of factors including: - concentration of Tetracyclin inducer (induces EutMN synthesis) - concentration of IPTG inducer (induces EutS synthesis) - Harvest time (time after induction) - Temperature - Growth Medium (LB and TB) / from this 22 flask investigation we were able to make surface plots to visulise our findings. /
- 2 TEMPERATURE DoE SURFACE PLOTS***
/ Figure1:
User Reviews
UNIQ6d6ded40ac2f7fe5-partinfo-00000000-QINU UNIQ6d6ded40ac2f7fe5-partinfo-00000001-QINU