Difference between revisions of "Part:BBa K911008"

Revision as of 19:40, 29 October 2017

Fast Germination (B.subtilis)

This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in the presence of L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.

This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 85
1000
COMPATIBLE WITH RFC[1000]

Users Review

2017 SZU-China iGEM team has changing the documented sequence above and create a new part as follow

BBa_K2232021

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 <partinfo>BBa_K911008 SequenceAndFeatures</partinfo>
-<span>This year, SZU-China</span>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K911008 parameters</partinfo>
 <!-- -->
 ===Users Review===
 SZU-China iGEM team has changing the documented sequence above and create a new part as follow
 <partinfo>BBa_K2232021</partinfo>