Difference between revisions of "Part:BBa K2271060:Experience"
 
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===Applications of BBa_K2271060===
 
===Applications of BBa_K2271060===
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For testing this part we used a fusion with the N-Terminus of a Peroxisomal Membrane anchor. We co-expressed this construct with Gus-PTS to perform a GUS Assay.(oder: We performed a GUS-Assay by targetting GUS(beta-Glucuronidase) to the Peroxisome using a GUS-PTS1.) The secreted GUS in the supernatant was measured with the turnover of 4-methylumbelliferyl-beta-D-glucuronide to 4-methyl umbelliferone (4-MUG). The fluorescent 4-MUG was measured with a plate reader (excitation: 365 nm, emission: 465 nm).
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Different peroxisomal membrane anchors were tested using the GUS-Assay. The highest activity of GUS could be measured in the supernatant of Pex15 (Link to part) as a Membrane Anchor.
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===User Reviews===
 
===User Reviews===
 
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Latest revision as of 21:21, 29 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2271060

User Reviews

UNIQ189163d54dc0571d-partinfo-00000000-QINU UNIQ189163d54dc0571d-partinfo-00000001-QINU