Difference between revisions of "Part:BBa K2232014"

Revision as of 18:19, 29 October 2017

P43_TSLV1-CA-alpha_rrnBT1

For facilitating the interconversion of CO₂ and HCO₃⁻ which is beneficial to the precipitation of CaCO₃, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The polyextremophilic bacterium Bacillus halodurans TSLV1 (MTCC 10961, 16S rDNA Acc. No. HQ235051) which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 851
Illegal NotI site found at 1138
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 831
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 376
Illegal SapI.rc site found at 534
Illegal SapI.rc site found at 846

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 <partinfo>BBa_K2232014 short</partinfo>
-For facilitating the interconversion of CO<sub>2</sub> and HCO<sub>3</sub><sup>−<sup> which is beneficial to the precipitation of CaCO<sub>3</sub>, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The polyextremophilic bacterium Bacillus halodurans TSLV1 (MTCC 10961, 16S rDNA Acc. No. HQ235051) which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.
+For facilitating the interconversion of CO<sub>2</sub> and HCO<sub>3</sub><sup>−</sup> which is beneficial to the precipitation of CaCO<sub>3</sub>, a plasmid containing Carbonic anhydrase coding gene have built and transferred into B. subtilis strain WB800 and express successfully.The promoter P43 used in the plasmid is a strong promoter in Bacillus subtilis 168,and the Carbonic anhydrase is from The polyextremophilic bacterium Bacillus halodurans TSLV1 (MTCC 10961, 16S rDNA Acc. No. HQ235051) which belong to α-type in classes of CAs. The terminator we used is rrnBT1(BBa_B0010) from E. coli rrnB, which will stop the Transcription of gene and raise the efficiency of expression.
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