Difference between revisions of "Part:BBa K2448021"

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The ''C. cellulolyticum'' Dpe is a 33.034 kDa protein of 293 amino acids.
 
The ''C. cellulolyticum'' Dpe is a 33.034 kDa protein of 293 amino acids.
  
This enzyme is highly specific for D-psicose, but is also able to catalyse with very low activity the epimerization of other D-tagatose [1].
+
This enzyme is highly specific for D-psicose, but it is also able to catalyse with very low activity the epimerization of D-tagatose [1].
  
 
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Revision as of 21:27, 29 October 2017


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

T--Evry Paris-Saclay--EC 5.1.3.30.png

The C. cellulolyticum Dpe is a 33.034 kDa protein of 293 amino acids.

This enzyme is highly specific for D-psicose, but it is also able to catalyse with very low activity the epimerization of D-tagatose [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 628
  • 1000
    COMPATIBLE WITH RFC[1000]