Difference between revisions of "Part:BBa K2448021"

Revision as of 15:14, 29 October 2017

D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-Psicose being the carbon-3 (C3) epimer of D-fructose.

The C. cellulolyticum Dpe is a 33.034 kDa protein of 293 amino acids.

This enzyme is highly specific for D-Psicose, but is also able to catalyse with very low activity the epimerization of other D-tagatose [1].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 628
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
-(Uniprot B8I944)
+(Uniprot B8I944).
 ===Usage and Biology===
+D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-Psicose being the carbon-3 (C3) epimer of D-fructose.
+[[File: T--Evry_Paris-Saclay--EC 5.1.3.30.png|300px]]
+The ''C. cellulolyticum'' Dpe is a 33.034 kDa protein of 293 amino acids.
+This enzyme is highly specific for D-Psicose, but is also able to catalyse with very low activity the epimerization of other D-tagatose [1].
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