Difference between revisions of "Part:BBa K2448007"

Latest revision as of 17:33, 1 November 2017

PsiR from Sinorhizobium fredii

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).

Our team used this transcription factor together with the Sinorhizobium fredii pPsiA or pPsiR promoters (BBa_K2448012 and BBa_K2448013) to build two Psicose biosensors (BBa_K2448028 and BBa_K2448029). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 691
Illegal AgeI site found at 1024
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
+PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).
 Our team used this transcription factor together with the ''Sinorhizobium fredii'' pPsiA or pPsiR promoters ([[Part:BBa_K2448012|BBa_K2448012]] and [[Part:BBa_K2448013|BBa_K2448013]]) to build two Psicose biosensors ([[Part:BBa_K2448028|BBa_K2448028]] and [[Part:BBa_K2448029|BBa_K2448029]]). The results show that PsiR behaved as predicted under and without induction.