Difference between revisions of "Part:BBa K2207010"
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This part is an inducible promoter regulated by PhlF. | This part is an inducible promoter regulated by PhlF. | ||
| − | It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas | + | It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens |
| + | and related species. | ||
In the absence of PhlF protein, this part promotes transcription. | In the absence of PhlF protein, this part promotes transcription. | ||
| + | |||
In the presence of PhlF protein, this part inhibits transcription. | In the presence of PhlF protein, this part inhibits transcription. | ||
| + | |||
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG). | PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG). | ||
| + | |||
PhlF can be coded by BBa_K2207009. | PhlF can be coded by BBa_K2207009. | ||
Revision as of 13:50, 29 October 2017
phlo Promoter
BBa_K2207010
Promoter(phlF regulated)
This part is an inducible promoter regulated by PhlF. It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.
In the absence of PhlF protein, this part promotes transcription.
In the presence of PhlF protein, this part inhibits transcription.
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
PhlF can be coded by BBa_K2207009.
Usage and Biology
The sequence of this part is designed and offered by Jef D. Boeke’s laboratory. And we used the mailed plasmid to test its function. As we can see from the figure below, it is obvious that this modified promoter can transcribe the CDS normally without the phlF in the Saccharomyces cerevisiaea.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 807
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]