Difference between revisions of "Part:BBa K2382012"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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A rigid linker link two proteins to form one fusion protein. It can prevent the
 
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two proteins from interrupting each other while folding.
 
−
 
  
 
The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
 
The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
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Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
 
Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
 
been successfully employed for many cases of protein purification without the tag of a fusion protein.
 
been successfully employed for many cases of protein purification without the tag of a fusion protein.
 +
 +
In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the
 +
two proteins from interrupting each other while folding.
  
 
===Characterization of the EAAAK===
 
===Characterization of the EAAAK===

Revision as of 12:49, 29 October 2017

EAAAK



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the target protein becomes automatically released from the fusion protein without proteolytic treatment. Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has been successfully employed for many cases of protein purification without the tag of a fusion protein.

In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding.

Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021