Difference between revisions of "Part:BBa K2382011"

(Usage and Biology)
Line 14: Line 14:
  
 
===Usage and Biology===
 
===Usage and Biology===
In order to have bacteria to produce antibody we have made a design.
+
This is the single chain variable fragment (scFv) of an antibody that have ability of binding
Since bacteria can’t produce the whole antibody, so we decide to use scFv to
+
aflatoxin. Since bacteria cannot produce the whole antibody, we decide to use scFv to replace it.
replace it. It contains two polypeptide chains linked by a linker.
+
It contains two polypeptide chains linked by a GS linker.
  
 
===Characterization of the Anti-antitoxin scFv===
 
===Characterization of the Anti-antitoxin scFv===
  
 
===References===
 
===References===

Revision as of 03:54, 30 October 2017

Anti-antitoxin scFv


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 339
    Illegal SapI.rc site found at 252


Usage and Biology

This is the single chain variable fragment (scFv) of an antibody that have ability of binding aflatoxin. Since bacteria cannot produce the whole antibody, we decide to use scFv to replace it. It contains two polypeptide chains linked by a GS linker.

Characterization of the Anti-antitoxin scFv

References