Difference between revisions of "Part:BBa K2374003:Design"
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According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly. | According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly. | ||
| − | <img | + | <img src=“https://static.igem.org/mediawiki/2017/c/cc/2017tongji_image_registry_UASTHtest.png” width="200px" height='200px" /> |
===Source=== | ===Source=== | ||
Revision as of 12:23, 29 October 2017
ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1157
Illegal XhoI site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 535
Illegal BsaI site found at 1501
Illegal BsaI.rc site found at 253
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 1168
Design Notes
site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG
According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.
<img src=“https://static.igem.org/mediawiki/2017/c/cc/2017tongji_image_registry_UASTHtest.png” width="200px" height='200px" />
Source
Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)
References
JANICE A. FISCHER, et al. GAL4 activates transcription in Drosophila. Nature 332, 853 - 856 (1988)