Difference between revisions of "Part:BBa K2325101"

Revision as of 12:01, 29 October 2017

Formamidase expression device

The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.

Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.

Usage and Biology

Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).

Figure 1. Growth curves of E.coli containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls

To verify that our recombinant E. coli containing formamidase gene could work efficiently to utilize formamide as its nitrogen sources, we cultivated the recombinant one and the negative controls in a basal MOPS medium with formamide as the sole nitrogen sources. E. coli containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While E.coli without formamidase gene basically did not grow.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 189
Illegal NgoMIV site found at 676
Illegal NgoMIV site found at 871
Illegal NgoMIV site found at 1018
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).
 <center>
-[[File:BBa_K2325101--1.png|600px]]<br />
+[[File:BBa_K2325101--1.png|500px]]<br />
 <b>Figure 1. Growth curves of <i>E.coli</i> containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls</b>
 </center>