Difference between revisions of "Part:BBa I732006"
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===Characterization T7 lacZalpha === | ===Characterization T7 lacZalpha === | ||
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<figure> | <figure> | ||
<img src="https://parts.igem.org/File:171002_CHARACTERIZATION_T7_LACZA_all.png" height="480" width="640"> | <img src="https://parts.igem.org/File:171002_CHARACTERIZATION_T7_LACZA_all.png" height="480" width="640"> | ||
<figcaption>Repeats of T7LacZalpha in T7M15 lysate</figcaption> | <figcaption>Repeats of T7LacZalpha in T7M15 lysate</figcaption> | ||
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====Improvements==== | ====Improvements==== | ||
This part was improved with a T7 promoter and terminator : BBa_K2203002 . | This part was improved with a T7 promoter and terminator : BBa_K2203002 . | ||
Revision as of 10:42, 29 October 2017
lacZ alpha fragment
In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization T7 lacZalpha
Improvements
This part was improved with a T7 promoter and terminator : BBa_K2203002 . This part is also used as a reporter in the following composite parts which are toehold switches : BBa_K2203003 , BBa_K2203004 , BBa_K2203005 , BBa_K2203006 .