Difference between revisions of "Part:BBa K309016:Experience"
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Test for GFP: localize the neuron-AWB | Test for GFP: localize the neuron-AWB | ||
We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron. | We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron. | ||
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| + | [[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png|800px|thumb|left|GFP expressed in AWB|GFP expressed in AWB]] | ||
Latest revision as of 16:24, 1 November 2017
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UNIQ8626674a2826b704-partinfo-00000000-QINU UNIQ8626674a2826b704-partinfo-00000001-QINU
Group: iGEM17_SUSTech_Shenzhen
Author: iGEM17_SUSTech_Shenzhen
Test for GFP: localize the neuron-AWB We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.
