Difference between revisions of "Part:BBa K2408023"

Revision as of 17:47, 29 October 2017

ADH1_Miraculin

This is a part improvment from BBa_K1033121 - team Uppsala 2013

Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag.

The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is a non-cariogenic substance, with a glycemic index of zero. It is suitable for consumption by diabetics and has the ability to convert sour taste into sweet tast. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of this special protein is still not entirely clear, but it is known that it is triggered in the presence low pH.

We added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1110
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 771
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1222
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1110
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1110
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 93

@@ Line 7: / Line 7: @@
 Miraculin optimized for <i>S. cerevisae</i> with the ADH1 promoter and histidine tag.
-The miraculin glycoprotein, originally produced from the <i>Synsepalum dulcificum</i> plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective.
+The miraculin glycoprotein, originally produced from the <i>Synsepalum dulcificum</i> plant, is a non-cariogenic substance, with a glycemic index of zero. It is suitable for consumption by diabetics and has the ability to convert sour taste into sweet tast. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of this special protein is still not entirely clear, but it is known that it is triggered in the presence low pH.
 We added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.