Difference between revisions of "Part:BBa K2448013"

Latest revision as of 21:41, 28 October 2017

pPsiR from Sinorhizobium fredii

This part is the promoter region (0.4 kb upstream) of the PsiR gene of Sinorhizobium fredii (NGR_b11520)

pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiR promoter together with the Sinorhizobium fredii PsiR transcription factor (BBa_K2448007) to build a Psicose biosensor (BBa_K2448029). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction.

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    350 LDF-  1.71
-10 box at pos.    335 gactatcct Score    43
-35 box at pos.    314 ttgcac    Score    33
Oligonucleotides from known TF binding sites:
No such sites for promoter at    350

Sequence and Features