Difference between revisions of "Part:BBa K2486025:Design"
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− | # Rangel-Chavez, C., Galan-Vasquez, E., & Martinez-Antonio, A. (2017). Consensus architecture of promoters and transcription units in Escherichia coli: design principles for synthetic biology. Molecular BioSystems, 13(4), 665-676. | + | #Rangel-Chavez, C., Galan-Vasquez, E., & Martinez-Antonio, A. (2017). Consensus architecture of promoters and transcription units in Escherichia coli: design principles for synthetic biology. Molecular BioSystems, 13(4), 665-676. |
Revision as of 18:57, 28 October 2017
16 base pair spacer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed this part to be used in situations where the CDS and terminator are synthesized together, this part would be designed in a smaller version if we used the iGEM 3A assembly, once there would be a scar between the CDS and the terminator.
Source
Escherichia coli K12 MG-1655
References
- Rangel-Chavez, C., Galan-Vasquez, E., & Martinez-Antonio, A. (2017). Consensus architecture of promoters and transcription units in Escherichia coli: design principles for synthetic biology. Molecular BioSystems, 13(4), 665-676.