Difference between revisions of "Part:BBa K2448008"

Revision as of 16:33, 28 October 2017

PsiR from Sinorhizobium meliloti

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium meliloti (SMb20483).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor together with the Sinorhizobium meliloti PsiA or PsiR promoters (BBa_K2448014, BBa_K2448015) to build two Psicose biosensors (BBa_K2448030 and BBa_K2448031). The results shows that PsiR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 691
Illegal AgeI site found at 1024
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.
-Our team used this transcription factor in some of our Psicose biosensors ([[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]) and the results shows that PsiR behaved as predicted under and without induction.
+Our team used this transcription factor together with the Sinorhizobium meliloti PsiA or PsiR promoters ([[Part:BBa_K2448014|BBa_K2448014]], [[Part:BBa_K2448015|BBa_K2448015]]) to build two Psicose biosensors ([[Part:BBa_K2448030|BBa_K2448030]] and [[Part:BBa_K2448031|BBa_K2448031]]). The results shows that PsiR behaved as predicted under and without induction.
 <!-- -->