Difference between revisions of "Part:BBa K2448001:Design"
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===References=== | ===References=== | ||
+ | |||
+ | [1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30. | ||
+ | |||
+ | [2] Addgene plasmid # 53976 |
Revision as of 14:21, 28 October 2017
mEmerald
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
Source
DNA synthesis
References
[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
[2] Addgene plasmid # 53976