Difference between revisions of "Part:BBa K2448004:Design"
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===Design Notes===
 
===Design Notes===
−
This codon optimized mCherry for Escherichia coli K12 is derived from ([[Part:BBa_J06504|BBa_J06504]]). The optimization has been performed with help of JCAT ([http://www.jcat.de/]).  
+
This codon optimized mCherry for Escherichia coli K12 is derived from ([[Part:BBa_J06504|BBa_J06504]]). The optimization has been performed with help of JCAT (http://www.jcat.de/).  
  
 
Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]])  
 
Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]])  

Revision as of 14:37, 28 October 2017


mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This codon optimized mCherry for Escherichia coli K12 is derived from (BBa_J06504). The optimization has been performed with help of JCAT (http://www.jcat.de/).

Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024)

Source

DNA synthesis

References