Difference between revisions of "Part:BBa K2271104:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The membrane domain | + | <html> |
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+ | The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by <a href="https://www.ncbi.nlm.nih.gov/pubmed/15133130"><abbr title="Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals"> Erdmann et al. (2004)</abbr></a>. We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest. | ||
+ | </p> | ||
+ | </html> | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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+ | <html><p> | ||
+ | Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann <i>et al.</i>, 2004 | ||
+ | </p> | ||
+ | </html> |
Latest revision as of 20:16, 1 November 2017
PEX13 membrane anchor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by Erdmann et al. (2004). We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest.
Source
UniProtKB - P80667 (PEX13_YEAST)
References
Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann et al., 2004