Difference between revisions of "Part:BBa K2448010"

Revision as of 20:19, 28 October 2017

pPsiA from Agrobacterium tumefaciens

This part is the promoter region (0.4 kb upstream) of the PsiA gene of Agrobacterium tumefaciens str. C58 (Atu4744)

pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448006) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiA promoter together with the Agrobacterium tumefaciens PsiR transcription factor (BBa_K2448006) to build a Psicose biosensor (BBa_K2448025). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction.

Using the BPROM webserver [2], the predict the -35 and -10 box sequences of this promoter are:

Threshold for promoters -  0.20
Promoter Pos:    117 LDF-  3.46
-10 box at pos.    102 tgatatggt Score    42
-35 box at pos.     83 ttgacg    Score    60
Oligonucleotides from known TF binding sites:
    rpoD16:  TAGAGCAC at position      67 Score -  10

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448010 short</partinfo>
-Promoter region (0.4 kb upstream) of PsiA gene of Agrobacterium tumefaciens str. C58 (Atu4744)
+This part is the promoter region (0.4 kb upstream) of the PsiA gene of ''Agrobacterium tumefaciens'' str. C58 (Atu4744)
+pPsiA is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448006|BBa_K2448006]]) which is inhibited in the presence of D-Psicose [1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Our team used this pPsiA promoter together with the ''Agrobacterium tumefaciens'' PsiR transcription factor ([[Part:BBa_K2448006|BBa_K2448006]]) to build a Psicose biosensor ([[Part:BBa_K2448025|BBa_K2448025]]). The results show that pPsiA is functional in ''E. coli'' and that it behaved as predicted under and without induction.
+Using the BPROM webserver [2], the predict the -35 and -10 box sequences of this promoter are:
+ Threshold for promoters -  0.20
+ Promoter Pos:    117 LDF-  3.46
+ -10 box at pos.    102 tgatatggt Score    42
+ -35 box at pos.     83 ttgacg    Score    60
+ Oligonucleotides from known TF binding sites:
+     rpoD16:  TAGAGCAC at position      67 Score -  10
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