Difference between revisions of "Part:BBa K2448015"

Revision as of 21:39, 28 October 2017

pPsiR from Sinorhizobium meliloti

This part is the promoter region (0.4 kb upstream) of the PsiR gene of Sinorhizobium meliloti (SMb20483)

pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiR promoter together with the Sinorhizobium meliloti PsiR transcription factor (BBa_K2448008) to build a Psicose biosensor (BBa_K2448031). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction.

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Promoter Pos:    317 LDF-  3.58
-10 box at pos.    302 gactatcat Score    53
-35 box at pos.    281 ttgcac    Score    33
Oligonucleotides from known TF binding sites:
       lrp:  TTTCTTTT at position     273 Score -   9
      ompR:  TTCTTTTT at position     274 Score -   8

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 179
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448015 short</partinfo>
-Promoter region (0.4 kb upstream) of PsiR gene of Sinorhizobium meliloti (SMb20483)
+This part is the promoter region (0.4 kb upstream) of the PsiR gene of ''Sinorhizobium meliloti'' (SMb20483)
+pPsiR is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Our team used this pPsiR promoter together with the ''Sinorhizobium meliloti'' PsiR transcription factor ([[Part:BBa_K2448008|BBa_K2448008]]) to build a Psicose biosensor ([[Part:BBa_K2448031|BBa_K2448031]]). The results show that pPsiR is functional in ''E. coli'' and that it behaved as predicted under and without induction.
+ Using the BPROM webserver [2], the predicted features of this promoter are:
+ Threshold for promoters -  0.20
+ Promoter Pos:    317 LDF-  3.58
+ -10 box at pos.    302 gactatcat Score    53
+ -35 box at pos.    281 ttgcac    Score    33
+ Oligonucleotides from known TF binding sites:
+        lrp:  TTTCTTTT at position     273 Score -   9
+       ompR:  TTCTTTTT at position     274 Score -   8
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