Difference between revisions of "Part:BBa K2486004"
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# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection].
 
# A promoter ([https://parts.igem.org/wiki/index.php/Part:BBa_J23108 BBa_J23108]) from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson promoter collection].
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# A ribosome binding site [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
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# A ribosome binding site ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]).
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# A short spacer of 6bp (gtccta)
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# A short spacer of 6bp (gtccta).
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# dtxR gene [https://parts.igem.org/Part:BBa_K2486005 BBa K2486005]
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# dtxR gene ([https://parts.igem.org/Part:BBa_K2486005 BBa K2486005]).
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# A short spacer of 16bp (ccagctcagtccgcat)
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# A short spacer of 16bp (ccagctcagtccgcat).
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# A synthetic terminator (BBa_K2486006)  
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# A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
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REFERENCES:
 
REFERENCES:

Revision as of 04:49, 28 October 2017


dtxR generator

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection.
  2. A ribosome binding site (BBa_B0034).
  3. A short spacer of 6bp (gtccta).
  4. dtxR gene (BBa K2486005).
  5. A short spacer of 16bp (ccagctcagtccgcat).
  6. A synthetic terminator (BBa_K2486006).

REFERENCES:

Boyd, J., Oza, M. N., & Murphy, J. R. (1990). Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proceedings of the National Academy of Sciences, 87(15), 5968-5972.

De Zoysa, A., Efstratiou, A., & Hawkey, P. M. (2005). Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. Journal of clinical microbiology, 43(1), 223-228.

https://www.wikigenes.org/e/gene/e/2649688.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]