Difference between revisions of "Part:BBa K2360012"

Revision as of 03:02, 28 October 2017

pChrB-ChrB+SRRz+rrnB terminater

It is a high-efficiency chromium detecting device. This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the chromium ion.

Figure 1. Bio-circuit of BBa_K2360012

We measured the growth curve of our engineering bacteria in different concentration of nickel ions. It was a pity that we found the OD600 of E. coli with did not decline after we added Cr⁶⁺, which showed this device did not work as we expected.

Figure 5. the growth curve of chromium detecting device in different concentration of chromium ions.

Sequence and Features