Difference between revisions of "Part:BBa K2360011"
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We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions.  
 
We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions.  
 
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[[File:SCUT_A PART PBS.PNG]]
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[[File:SCUT_A PART PB S.PNG]]
 
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Figure  4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH<sub>2</sub>O
 
Figure  4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH<sub>2</sub>O

Revision as of 02:52, 28 October 2017


rrnB' terminater+PbrR+pPbrR+SRRz+rrnB terminater

It is a high-efficiency lead detecting device. This device consist two parts, the lysis module (BBa_K2360000) and lead detecting module(BBa_K2360006).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the lead ion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]