Difference between revisions of "Part:BBa K2267045"

Latest revision as of 19:33, 1 November 2017

LuxR-I-Plux-GFP-8

This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.

Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (part1: https://parts.igem.org/Part:BBa_K2267033 ) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.

 https://parts.igem.org/Part:BBa_K2267040  is Control，

part1: https://parts.igem.org/Part:BBa_K2267043

part3: https://parts.igem.org/Part:BBa_K2267041

part4: https://parts.igem.org/Part:BBa_K2267042

part6: https://parts.igem.org/Part:BBa_K2267044

part8: https://parts.igem.org/Part:BBa_K2267045

result

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 928
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2179

@@ Line 10: / Line 10: @@
 <!-- -->
 Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
-In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
+In this case, the constitutively expressed LuxR transcriptional regulator (part1:  https://parts.igem.org/Part:BBa_K2267033 ) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
    https://parts.igem.org/Part:BBa_K2267040  is Control，
-part1: https://parts.igem.org/Part:BBa_K2267026  https://parts.igem.org/Part:BBa_K2267029  https://parts.igem.org/Part:BBa_K319039.
-part3: https://parts.igem.org/Part:BBa_K2267026   https://parts.igem.org/Part:BBa_K2267030  https://parts.igem.org/Part:BBa_K319039.
-part4: https://parts.igem.org/Part:BBa_K2267026   https://parts.igem.org/Part:BBa_K2267031  https://parts.igem.org/Part:BBa_K319039.
-part6: https://parts.igem.org/Part:BBa_K2267026   https://parts.igem.org/Part:BBa_K2267032  https://parts.igem.org/Part:BBa_K319039.
-part8: https://parts.igem.org/Part:BBa_K2267026   https://parts.igem.org/Part:BBa_K2267033   https://parts.igem.org/Part:BBa_K319039.
 part1:  https://parts.igem.org/Part:BBa_K2267043