Difference between revisions of "Part:BBa K2267041"

Revision as of 22:43, 27 October 2017

LuxR-I-Plux-GFP-3

This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.

Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.

https://parts.igem.org/Part:BBa_K2267040  is Control， 

part1: https://parts.igem.org/Part:BBa_K2267026 https://parts.igem.org/Part:BBa_K2267029 https://parts.igem.org/Part:BBa_K319039.

part3: https://parts.igem.org/Part:BBa_K2267026 https://parts.igem.org/Part:BBa_K2267030 https://parts.igem.org/Part:BBa_K319039.

part4: https://parts.igem.org/Part:BBa_K2267026 https://parts.igem.org/Part:BBa_K2267031 https://parts.igem.org/Part:BBa_K319039.

part6: https://parts.igem.org/Part:BBa_K2267026 https://parts.igem.org/Part:BBa_K2267032 https://parts.igem.org/Part:BBa_K319039.

part8: https://parts.igem.org/Part:BBa_K2267026 https://parts.igem.org/Part:BBa_K2267033 https://parts.igem.org/Part:BBa_K319039.

part1: https://parts.igem.org/Part:BBa_K2267043

part3: https://parts.igem.org/Part:BBa_K2267041

part4: https://parts.igem.org/Part:BBa_K2267042

part6: https://parts.igem.org/Part:BBa_K2267044

part8: https://parts.igem.org/Part:BBa_K2267045

result

Sequence and Features