Difference between revisions of "Part:BBa K592022"
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2017 Team NYU Abu Dhabi attempted to express cjBlue in DH5-α using composite part BBa_K592022. Our assembly and transformation [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocols] were followed in order to obtain the part. All initial, intermediate, and final products obtained throughout the 3A assembly were digested with EcoRI-HF following our digestion [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocol] and run in a 1.2% agarose gel to confirm that the constructs were of the right size (Figure 1). | 2017 Team NYU Abu Dhabi attempted to express cjBlue in DH5-α using composite part BBa_K592022. Our assembly and transformation [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocols] were followed in order to obtain the part. All initial, intermediate, and final products obtained throughout the 3A assembly were digested with EcoRI-HF following our digestion [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocol] and run in a 1.2% agarose gel to confirm that the constructs were of the right size (Figure 1). | ||
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"https://static.igem.org/mediawiki/2017/a/a9/Gel_Result_cjBlue_NYUAD2017.png" | "https://static.igem.org/mediawiki/2017/a/a9/Gel_Result_cjBlue_NYUAD2017.png" | ||
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However, no blue colonies were observed after 24 hs of incubation in agar and no blue pellet was obtained after centrifuging an overnight LB broth culture at 7000 rpm for 5 min. The reason for the lack of expression remains elusive. | However, no blue colonies were observed after 24 hs of incubation in agar and no blue pellet was obtained after centrifuging an overnight LB broth culture at 7000 rpm for 5 min. The reason for the lack of expression remains elusive. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 22:13, 27 October 2017
cjBlue Green Coding Device
This part is constitutively expressed BBa_K592011, the cjBlue chromoprotein. The color development is slow, it is readily visible to naked eye after 24-48 hours of incubation.
Escherichia coli constitutively expressing cjBlue (BBa_K592011).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
2017 Team NYU Abu Dhabi attempted to express cjBlue in DH5-α using composite part BBa_K592022. Our assembly and transformation [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocols] were followed in order to obtain the part. All initial, intermediate, and final products obtained throughout the 3A assembly were digested with EcoRI-HF following our digestion [http://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols protocol] and run in a 1.2% agarose gel to confirm that the constructs were of the right size (Figure 1).
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However, no blue colonies were observed after 24 hs of incubation in agar and no blue pellet was obtained after centrifuging an overnight LB broth culture at 7000 rpm for 5 min. The reason for the lack of expression remains elusive.