Difference between revisions of "Part:BBa K611016"
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For 2017, Team UAlberta submitted a new part, <a href="https://parts.igem.org/Part:BBa_K2389070)BBa_K2389070</a>. In this construct, the promoter for LacI was replaced with a constitutive promoter, <a href="https://parts.igem.org/Part:BBa_J23106">BBa_J23106</a>, which eliminates the need to add arabinose as an extra additive.
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For 2017, Team UAlberta submitted a new part, <a href="https://parts.igem.org/Part:BBa_K2389070>BBa_K2389070</a>. In this construct, the promoter for LacI was replaced with a constitutive promoter, <a href="https://parts.igem.org/Part:BBa_J23106">BBa_J23106</a>, which eliminates the need to add arabinose as an extra additive.
 
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Revision as of 22:00, 27 October 2017


LacI Repressor and Promoter Wild Type Control

This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.

For 2017, Team UAlberta submitted a new part, BBa_J23106, which eliminates the need to add arabinose as an extra additive.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2394
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870